Phytochemical and Biological Characterization of Aqueous and Ethanolic Extracts of Parthenium hysterophorus

Introduction: Parthenium hysterophorus is a plant used in traditional medicine to treat health issues and which could be source of phytochemicals with possible antioxidant activity without causing cytotoxic effects. Hence, this work was designed evaluate its phytochemical profile, cytotoxicity, activity. Methods: The aqueous (AE) ethanolic (EE) extracts P. flowers were obtained by decoction ultrasound, respectively. Their composition determined colorimetric tests RP-HPLC-MS analysis. tested hemolysis assay. evaluated the Trolox equivalent capacity (TEAC), 2,2-diphenyl-1- picrylhydrazyl (DPPH), hydroxyl radical (-OH) scavenging assays. In addition, effect on enzymes superoxide dismutase (SOD) catalase (CAT) from human erythrocytes, evaluated. Results: screening AE EE test showed presence flavonoids, steroids, triterpenes, saponins, coumarins, sesquiterpene lactones, tannins, carbohydrates. analysis identified some phenolic compounds such as flavonols, methoxyflavonols, flavones, methoxyflavones, hydroxycinnamic acids. assay non-cytotoxic AE, but exhibited hemolytic effect. Furthermore, significant inhibit radicals TEAC, DPPH -OH Moreover, SOD only increase AE. However, two crude increased CAT activity, at highest concentrations. Conclusion: has vitro.